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Factors to Consider When Extracting DNA Molecule
The most important component in the human’s blood is the DNA molecule and is the first thing looked into incase of any problem. The identification of any child is the DNA since it is through that is when the parents can be traced and mistakes cannot be made since the DNAs can never be similar. The identification mark for any wanted person in the current society is the use of the DNA and it can be traced from the fingerprints of a person. The cell normally a lot of components and to get each one of them, separation has to be made. Experts are the only ones doing the exercise since it is technical and many factors have to be considered for the correct desired results to be made.
All the tools used in the extraction have to be purely clean and even when doing the extraction to avoid minor mistakes that might cost the whole activity. Methods of extracting it are three and the most suitable one can be used, the incubation method has to be done overnight, the reagent one which is newly installed and there is the protocol type which is the easiest method completed in just some few minutes. Despite every one having a right to choose on what to do, there are circumstances that forces imposition of certain methods and the whole activity to be do and failing to follow that can lead to other problems.
Every component has its own unique features and becomes only possible to identify them through them without confusing with the rest. The negative charges of the DNA molecule make it possible for them to be identified from the rest of the molecules. They tend to coil around protein molecules that are positively charged and are in a coiled formed because of the chromosomes they have.
The steps in the DNA extraction are three and have to be followed systematically to acquire the exact results. The whole cell membrane has to be raptured for the components to be released and separated for each and every component to be on its own. The exercise can only be done well and perfectly with the cell opened. To open up the cell membrane, one can use heat to increase the fluidity state or some specified detergents that softens it making the operation easy.
DNA features play the role of proper separation without confusion. Due to the fact that some features might be similar for some of the different molecules, a lot of carefulness has to be considered. Isolation is the final step made if the DNA molecule has been extracted for the wanted use.